Comprehensive Guide to Mouse ISG15 (Ubiquitin-like Protein) and Its ELISA Kit Applications

Mouse ISG15 (Interferon-Stimulated Gene 15) is a ubiquitin-like protein that plays a crucial role in immune responses, particularly in antiviral defense mechanisms. This protein is part of the ISGylation pathway, a post-translational modification process that helps regulate the immune response and other cellular processes. The Mouse ISG15 ELISA Kit is an essential tool for detecting and quantifying ISG15 levels in various biological samples, aiding researchers in understanding its functions and implications in disease

Biological Significance of ISG15

ISG15 is induced by type I interferons (IFN-α/β) and is involved in several key processes:

  1. Antiviral Immunity: ISG15 plays a central role in the innate immune response to viral infections by modulating protein activity through ISGylation.
  2. Cellular Stress Responses: ISG15 expression is upregulated in response to stress signals, including viral infections and inflammatory cytokines.
  3. Cancer Research: Aberrant ISGylation has been implicated in tumor progression and immune evasion.

For further insights into ISG15 biology, visit PubMed and the National Center for Biotechnology Information (NCBI).

What is the ISG15 ELISA Kit?

The ISG15 ELISA Kit is a highly sensitive and specific assay designed to detect ISG15 levels in biological samples such as serum, plasma, cell culture supernatants, and tissue lysates. This kit leverages the enzyme-linked immunosorbent assay (ELISA) technology for accurate and reproducible results.

Features of the ISG15 ELISA Kit:

  • High Sensitivity: Detects low concentrations of ISG15.
  • Specificity: Uses highly specific antibodies to minimize cross-reactivity.
  • Versatility: Suitable for various sample types.
  • Convenience: Ready-to-use reagents with optimized protocols.

For technical guidance, explore resources provided by the National Institutes of Health (NIH) and NIH RePORTER.

Applications of the ISG15 ELISA Kit

1. Immunology and Infectious Diseases

ISG15’s critical role in antiviral immunity makes it a valuable biomarker for:

  • Investigating host responses to viral infections.
  • Monitoring immune activation in diseases such as COVID-19, influenza, and hepatitis.
  • Understanding cytokine signaling pathways.

For additional information, refer to the National Institute of Allergy and Infectious Diseases (NIAID).

2. Cancer Research

ISG15’s dual role in cancer biology—both as a tumor suppressor and a promoter—is of significant interest. The ELISA kit is used to:

  • Quantify ISG15 levels in tumor microenvironments.
  • Study its interaction with immune checkpoints.
  • Evaluate the effects of immunotherapy on ISGylation.

Explore cancer research at the National Cancer Institute (NCI).

3. Autoimmune and Inflammatory Diseases

ISG15 is upregulated in autoimmune conditions such as systemic lupus erythematosus (SLE). The ELISA kit can help in:

  • Identifying ISG15 as a biomarker for disease severity.
  • Investigating its role in cytokine storms and inflammation.
  • Developing targeted therapies for autoimmune disorders.

Learn more from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS).

How the ISG15 ELISA Kit Works

The ISG15 ELISA Kit follows a sandwich ELISA principle:

  1. Coated Wells: Microplate wells are pre-coated with a capture antibody specific to ISG15.
  2. Sample Incubation: Samples or standards are added to the wells, allowing ISG15 to bind to the capture antibody.
  3. Detection Antibody: A biotinylated detection antibody specific to ISG15 is added, followed by an enzyme-conjugated streptavidin.
  4. Signal Development: A substrate solution is added to develop a colorimetric signal proportional to ISG15 concentration.
  5. Quantification: The optical density (OD) is measured, and the ISG15 concentration is determined using a standard curve.

Detailed protocols can be found at the National Institute of Standards and Technology (NIST).

Advancing Research with the ISG15 ELISA Kit

The ISG15 ELISA Kit is pivotal in unraveling:

  1. Immune Response Mechanisms: Understanding how ISG15 modulates antiviral and inflammatory pathways.
  2. Biomarker Discovery: Identifying ISG15 as a diagnostic or prognostic marker in infections and cancer.
  3. Therapeutic Development: Exploring ISG15-targeted interventions in immunotherapy and antiviral treatments.

For more on recent developments, visit ClinicalTrials.gov and NIH Funding Opportunities.

Supporting Resources

Enhance your research with these resources:

Conclusion

The Mouse ISG15 ELISA Kit is a versatile and reliable tool for advancing our understanding of ISG15’s role in immunity, cancer, and inflammation. By providing precise and reproducible data, it supports researchers in identifying novel therapeutic targets and improving disease management strategies.

Explore additional resources and funding opportunities at NIH, FDA, CDC, and NIEHS. For specific inquiries or technical support, connect with research communities or browse guides on platforms like ResearchGate.

 


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