Mouse 12-Hydroxyeicosatetraenoic Acid (12-HETE) is a significant eicosanoid metabolite derived from arachidonic acid. This lipid molecule is synthesized by the enzymatic action of 12-lipoxygenase and has been widely studied for its involvement in diverse physiological and pathological processes, including inflammation, cancer progression, and vascular regulation. This article provides an in-depth look at 12-HETE, its biological relevance, and the applications of the 12-HETE ELISA Kit in research.
Biological Significance of 12-HETE
12-HETE is part of the hydroxyeicosatetraenoic acids (HETEs) family and is involved in cellular signaling. It is known to regulate:
- Inflammatory Responses: 12-HETE plays a crucial role in the modulation of cytokine release, leukocyte migration, and inflammatory cascades, making it a key player in immune responses.
- Tumor Microenvironment: Studies reveal that 12-HETE promotes angiogenesis and cancer cell proliferation, acting as a mediator in the tumor microenvironment.
- Vascular Function: It contributes to vasoconstriction and endothelial cell signaling, influencing cardiovascular health.
For additional insights, researchers can explore authoritative databases like PubMed and the National Cancer Institute for peer-reviewed literature on 12-HETE
What is the 12-HETE ELISA Kit?
The 12-HETE ELISA Kit is a highly specific and sensitive assay designed to detect and quantify 12-HETE in various biological samples, including serum, plasma, and tissue lysates. This technology leverages the principles of enzyme-linked immunosorbent assay (ELISA), allowing researchers to measure 12-HETE levels accurately.
Features of the 12-HETE ELISA Kit:
- High Sensitivity: Detects low levels of 12-HETE in complex samples.
- Specificity: Designed to minimize cross-reactivity with other eicosanoids.
- Versatility: Compatible with a wide range of sample types.
- Ease of Use: Provides ready-to-use reagents and standardized protocols.
For further technical details, refer to resources provided by the National Center for Biotechnology Information (NCBI) and NIH RePORTER.
Applications of the 12-HETE ELISA Kit
1. Inflammation Research
12-HETE’s role in inflammatory pathways is well-documented. It has been implicated in diseases such as asthma, rheumatoid arthritis, and inflammatory bowel disease. The ELISA kit enables precise quantification of 12-HETE to investigate:
- Cytokine regulation.
- Immune cell activation.
- The interaction between eicosanoids and other inflammatory mediators.
Explore studies on inflammation at the National Institute of Allergy and Infectious Diseases (NIAID).
2. Cancer Studies
12-HETE’s involvement in cancer progression includes promoting tumor growth, metastasis, and angiogenesis. Researchers can:
- Monitor 12-HETE as a biomarker for cancer progression.
- Study its interaction with oncogenic pathways.
- Evaluate the impact of therapeutic interventions targeting 12-HETE.
For resources, visit the National Cancer Institute (NCI) and the Food and Drug Administration (FDA).
3. Cardiovascular Research
12-HETE influences vascular tone, endothelial function, and platelet aggregation. This makes it a critical molecule in studies of:
- Hypertension.
- Atherosclerosis.
- Stroke and other cardiovascular conditions.
Find more on cardiovascular research at the National Heart, Lung, and Blood Institute (NHLBI).
4. Metabolic Disorders
The role of 12-HETE in insulin resistance and metabolic syndrome is gaining attention. The ELISA kit aids in evaluating:
- Insulin signaling pathways.
- β-cell dysfunction in diabetes.
- Lipid metabolism.
Visit the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) for further insights.
How the 12-HETE ELISA Kit Works
The 12-HETE ELISA Kit utilizes a competitive binding method:
- Sample Preparation: Biological samples are prepared and added to microplate wells coated with a 12-HETE-specific antibody.
- Competitive Binding: A fixed amount of biotinylated 12-HETE competes with the sample’s 12-HETE for antibody binding sites.
- Detection: After washing, an enzyme-linked secondary antibody is added, followed by a substrate to produce a measurable signal proportional to 12-HETE levels.
- Quantification: The signal is read using a spectrophotometer, and the results are calculated against a standard curve.
For detailed methodologies, refer to the National Institute of Standards and Technology (NIST).
Advancing Research with the 12-HETE ELISA Kit
The ELISA kit has been a cornerstone in biomedical research, enabling breakthroughs in:
- Pathogenesis Studies: Understanding disease mechanisms involving 12-HETE.
- Therapeutic Development: Evaluating the efficacy of drugs targeting eicosanoid pathways.
- Biomarker Discovery: Identifying 12-HETE as a diagnostic or prognostic marker for diseases.
For up-to-date research and funding opportunities, explore NIH Funding Resources and ClinicalTrials.gov.
Supporting Resources
To further explore the role of 12-HETE in research, consider the following resources:
- National Institute of Environmental Health Sciences (NIEHS): Resources on eicosanoids and toxicology.
- Environmental Protection Agency (EPA): Data on lipid mediators in environmental health.
- Centers for Disease Control and Prevention (CDC): Guidelines for studying biomarkers in public health.
- National Toxicology Program (NTP): Comprehensive toxicological profiles.
The Mouse 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit is an indispensable tool for researchers aiming to uncover the molecular intricacies of inflammation, cancer, cardiovascular health, and metabolic disorders. By providing precise and reliable data, it empowers scientists to push the boundaries of knowledge and develop innovative therapeutic strategies.
Explore additional resources and support from academic and government institutions such as NIH, FDA, CDC, and NIEHS.
For further inquiries about the ELISA kit, consult technical guides or connect with research communities on platforms like ResearchGate.
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